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mouse anti syntaxin1a  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank mouse anti syntaxin1a

    Mouse Anti Syntaxin1a, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+syntaxin1a/anti-Syntaxin/pmc11513836-486-18-21
    Average 94 stars, based on 61 article reviews
    mouse anti syntaxin1a - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "A candidate loss-of-function variant in SGIP1 causes synaptic dysfunction and recessive parkinsonism"

    Article Title: A candidate loss-of-function variant in SGIP1 causes synaptic dysfunction and recessive parkinsonism

    Journal: Cell Reports Medicine

    doi: 10.1016/j.xcrm.2024.101749


    Figure Legend Snippet:

    Techniques Used: Recombinant, Bradford Protein Assay, Sequencing, Cloning, Software, Functional Assay, Imaging

    Related Articles

    other:

    Article Title: Drosophila Wingless is loaded on exosome-like vesicles but forms a gradient in an exosome-independent manner
    Article Snippet: Primary antibodies used were: anti-Wingless (1:1000; Developmental Studies Hybridoma Bank, DSHB), anti-Wnt3A (1:500; Cell Signaling Technologies), anti-Flotillin2 (1:1000; BD Transduction Laboratories), anti-Tsg101 (1:500; Abcam), anti-actin (1:1000; DSHB), anti-Evi (1:20,000; kindly provided by K. Basler), anti-GPR177 (1:2500; Lifespan Biosciences), anti-Syntaxin1A (1:500; DSHB), anti-Csp (1:1000; DSHB), anti-Rab35 (1:5000; Arnaud Echard), anti-HrsFL (1:20,000; kindly provided by H. Bellen) and anti-Vps28 (1:5000; Helmut Kramer).

    Article Title: Postsynaptic Membrane Addition Depends on the Discs-Large-Interacting t-SNARE Gtaxin
    Article Snippet: To probe the blots, the following antibodies were used: anti-GTX (1:5000), rat antiDLG (1:5000) (Koh et al., 1999), or mouse anti-DLG (1:2000, Developmental Studies Hybridoma Bank), anti-Tubulin (1:4000; Sigma), mouse anti- Spectrin (3A9, 1:1000; Developmental Studies Hybridoma Bank), and anti-Syntaxin1A (8C3, 1:100; Developmental Studies Hybridoma Bank).

    Incubation:

    Article Title: A genetic and physiological model of renal dysfunction in Lowe syndrome
    Article Snippet: Samples were separated by SDS-Polyacrylamide gel electrophoresis and transferred onto nitrocellulose filter membrane [Hybond-C Extra; (GE Healthcare, Buckinghamshire, UK)] using wet transfer (BioRad, California, USA). .. Membranes were blocked with 5% Blotto (sc-2325, Santa Cruz Biotechnology, Texas, USA) in PBS with 0.1% Tween 20 (Sigma Aldrich) (PBST) for 2 h at room temperature (25 ° C) followed by incubation in primary antibody diluted in 5% Blotto in PBST overnight at 4 0 C. Primary antibodies dilutions used were 1:1000 anti-dOCRL (Avital Rodal lab), 1:1000 anti-HA (CST, #2367S), 1:4000 anti-α-tubulin-E7c (DHSB, lowa, USA), 1:1000 anti-Syntaxin1A (DSHB, Iowa, USA), 1:3000 anti-RFP (Rockland, 600-401-600-RTU), 1:1000 anti-α-actin (Sigma Aldrich), 1:1000 anti-mcherry (Thermo, PA5-34974) 1:2000 anti-GFP (Santa cruz, sc9996). .. Following this, the membrane was washed for 10 minutes thrice with PBST and incubated with the appropriate HRP conjugated secondary antibody (Jackson Laboratories, USA) at 1:10000 dilution in PBST for 2 hours at room temperature.



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    Image Search Results


    Journal: Cell Reports Medicine

    Article Title: A candidate loss-of-function variant in SGIP1 causes synaptic dysfunction and recessive parkinsonism

    doi: 10.1016/j.xcrm.2024.101749

    Figure Lengend Snippet:

    Article Snippet: The following antibodies were used: guinea pig anti-EndoA (GP69) [1:2000], rabbit anti-GFP [1:1000 (Invitrogen)], mouse anti-Brp [1:50 (DSHB)], mouse anti-Syntaxin1A [1:50 (DSHB)], mouse anti-CSP [1:50 (DSHB)], mouse anti-GluRIIA [1:100 (DSHB)], mouse anti-Synaptotagmin1 [1:50 (DSHB)], rabbit anti-Synaptojanin [1:500], guinea pig anti-Vha100-1 [1:2000 (gift from Robin Hiesinger ], mouse anti-Synapsin [1:100 (DSHB)], mouse anti-Dynamin [1:500 (BD Biosciences)], rat anti-Synaptobrevin [1:1000 (gift from Hugo Bellen)], mouse anti-DLG [1:50 (DSHB)], rabbit anti-HRP [1:1000 (Jackson ImmunoResearch)], rabbit anti-TH [1:200 (Millipore)].

    Techniques: Recombinant, Bradford Protein Assay, Sequencing, Cloning, Software, Functional Assay, Imaging